Science

Introduction When casein paint paint (a protein in milk) is hydrolysed, the milk turns from cloudy to clear. Trypsin is unity of the enzymes up to(p) to do this. Aim To investigate the effect of temperature on the practise of trypsin - exploitation casein as the substratum. Materials and method A piss bathing tub was heat to 25°C. 5cm³ of 4% casein solution was put into one test pipage and 5cm³ of 0.5% trypsin was added to other test tube. Both were stood in a water system bath until they reached the correct temperature. The same measuring of casein solution was added to another(prenominal) test tube and 5cm³ distilled water in a fourth test tube. This acted as a control. Another control consisted of 5cm³ distilled water in a twenty percent test tube and 5cm³ casein in a lowest test tube. each of these were also fixed into the water bath. sear card was placed behind the test tubes to help tarnish the clearing of the solution. The enzyme and sub strate were mixed, a stopwatch started immediately, and the time for the severance to clear tell. This was tell for the controls, and the whole experiment repeated for antithetic temperatures, ranging from 25°C - 65°C. Results and discussion The control showed no transfigure in chroma at any temperature.

In the interpret below, we plan the relative reaction rate (1/time taken to dole out a colour shift) against the temperature. As the temperature increased, the time for a colour change to be seen was less (1/t was bigger). At a certain point, the relative reaction rate started decreasing. This was t he optimal temperature (~ 37°C). Experi! ment to determine the rate of reaction of trypsin on casein Click on the next step button to trace the colour change. Graph depicting the effect of temperature on trypsin activity accomplishable errors The timing may cave in been inaccurate.The colour change is subjective. This essence that each person will decide differently when they see a colour change and so everyone would have noted a different time.If you want to get a beneficial essay, order it on our website: OrderCustomPaper.com

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