Microbiology: Viable Counts

Introduction Total Viable itemize is a decimal idea about the presence of microorganisms such as bacterium, barm and mold in a sample. It counts the quash of colonies produced by a in truth dilute suspension of bacteria on an agar collection plateful and to observe the differential blot behaviour of the living bacteria. This involves counting the colonies produced by possible cells at a lower place favourable growth conditions. some(a) techniques needed before the viable count, like pour plate regularity, spread plate method and most probable payoff method. The viable count is very specidic, as it represents the result of habituation forming units (/g) or (/ml) of the sample. B) VIABLE COUNTS Two methods of acquire viable counts argon available i. Spread plate method. ii. Most probable number (MPN) method. (i) Spread Plate order (Lawn Culture) Materials: Dilution series prepared in (A) Sterile 1ml pipette 6 intellectual nourishment agar (NA) plates 1 crackpot field hockey stick 1 beaker of alcohol Procedure All move should be make using aseptic technique The culture tagged 10-8 was mildly mixed. 0.1ml of this dilution was aseptically transferred onto the center of a NA plate.

Bend end of the glass in spreader was dipped in alcohol, then it was sterilized by flaming and allowed to unruffled down. The glass hockey stick was use to spread the sample over the aerofoil of the plate. It was an even distribution. This outgrowth was repeated using a second NA plate. These plates were designate as 10-8. So, du plicated plate was counted. Steps 1,2 an! d 3 was repeated for dilution 10-7 and 10-6 in order. 6 plates was prepared and 2 at each of the dilution 10-8, 10-7 and 10-6. The plates were incubated at 32oC for about 2 days. The number of colonies on... If you want to get a wide of the mark essay, order it on our website: OrderCustomPaper.com

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